Rhizomal buds of Angelica sinensis (Oliv.) Diels were collected from a field in Kunming Institute of Botany, China. Rhizomal buds were sterilized with orthocide and Clorox. The buds were removed aseptically from the rhizome and cultured onto Murashige and Skoog (MS) basal medium. After one month, shoot tips of plantlets induced from the buds were subcultured onto MS containing 3 mg/l benzyl amino purine (BAP or B) and 0.1 or 1 mg/l naphthalene acetic acid (NAA), and MS58 (0.1 mg/l BAP, 5 mg/l giberellic acid, 150 mg/l citric acid and double the strength of iron) for multiple shoot induction. The best media for multiple shoot induction were MS containing 3 mg/l BAP and 1 mg/l NAA which had an average of 3.45 shoots per culture in one month. The rooting medium was MS containing 0.2 mg/l BAP and 2 mg/l 3-indoleacetic acid (IAA), 0.1 mg/l kinetin (KN) and 0.02 mg/l NAA, and filter paper moistened with 1 MS liquid medium. The result of the best rooting medium was 0.1 mg/l KN and 0.02 mg/l NAA which became plantlets. The percentage of survival for plantlets grown in a tissue culture room (controlled temperature = 25°C, with 16 hours of light) was 100 per cent after one and two months, compared with those grown in a nursery, which were 83.5 and 72.6 percent, respectively.

Key words: Angelica sinensis, tissue culture